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Joung and colleagues have established a bacterial assay,20 which could be adapted by electroporating synthetically generated crXNAs into bacterial cells inducibly expressing Cas9 variants libraries.
The bacterial assay was affected by toxicant contaminated water for an induction time that ranged between 30 min and 120 min.
The PEG coated surfaces showed excellent antifouling properties upon exposure to human blood and bacteria as demonstrated by fluorescence microscopy, ellipsometry and a bacterial assay with Staphylococcus epidermidis.
They are also switching, where feasible, to a variety of alternatives: tissue cultures to test and produce some vaccines, the Ames bacterial assay to screen for mutagens, computer models to mimic biological processes, nuclear magnetic resonance to visualize internal organs without cutting into an animal.
The bacterial assay was performed by agar well diffusion method [26].
Bacterial assay (live and dead cell) was performed by using gram negative E. coli strains after 6 h post treatment with synthesized silver nanoparticles using Acridine orange (AO) as staining dye.
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Of all the Acrs tested in human cells and in the bacterial assays, AcrIIA5 displayed the broadest range of activity (Figs. 4, 5).
To perform the activity labelling, two matrices are constructed (one for biochemical assays and one for bacterial assays).
Second, we applied 11 bacterial assays; but this does not represent all bacterial taxa associated with the vagina.
To quantify the disease symptom, bacterial assays were carried out to measure the titer of bacterial on infected leaves.
Additional release profiles and the results of additional bacterial assays (PDF ) Additional release profiles and the results of additional bacterial assays (PDF) § Y.T. is currently at Department of Chemistry, University of Nevada, Reno, 1664 N. Virginia Street, Reno, NV 89557.
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