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One hour after incubation, bacteria were washed out and medium was changed with one containing antibiotics (gentamycin 100 µg/mL).
Subsequently bacteria were washed out and cells were left in culture for an additional 23 h in medium containing gentamycin 100 µg/mL.
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In our study, the Bacteroidetes bacterium was washed out under SBR operational conditions, but retained under A/O and OD operational conditions due to the longer settling time.
Septic tanks under beachfront homes were washed out by storms in 1983, sending bacteria into the water and polluting 12 miles of the coastline.
After this process, the unbound bacteria in the root canals of the remaining 45 teeth then were washed out with buffer until baseline levels of radioactivity were obtained.
Former works had proved that the Bacteroidetes bacterium was washed out at short settling times and did not contribute to sludge granulation [ 33].
To remove unbound Vn, bacteria were washed twice in PBS.
For BCG infections, bacteria were washed in complete media without antibiotics, and then APCs were inoculated at the designated MOI.
The bacteria were washed and diluted in sterile water.
After extensive HBSS washing, the extracellular bacteria were washed away.
Prior to use, bacteria were washed three times with PBS.
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