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Macrophages in chamber slides were fixed with 4% paraformaldehyde and intracellular acid-fast bacteria were visualized using Kinyoun stain.
For fluorescence analysis, bacteria were visualized using a Nikon Eclipse TE300 (Nikon, Melville, NY) inverted fluorescence microscope equipped with a Hamamatsu C4742-95 CCDled cameramera (Hamamatsu, Japan) and a Plan Apochromat ×100 objective (Olympus, Orangeburg, NY).
Nuclei and bacteria were visualized using DAPI staining.
Bacteria were visualized using a Nikon Eclipse TE300 (Nikon, Melville, USA) inverted fluorescence microscope equipped with a Hamamatsu C4742-95 CCDled cameramera (Hamamatsu, Bridgewater, USA) and a Plan Apochromat ×100 objective (Olympus, Orangeburg, USA).
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Cuticle preps were visualized using dark field microscopy.
Amplified PCR fragments were visualized using 1.5% agarose gels.
Cell cultures were visualized using phase-contrast microscopy at each timepoint and images captured.
PCR products were visualized using 1.0% agarose gels (1X TAE) stained with Gelstar®.
Skyline plots were visualized using EXCEL.
The protein bands were visualized using NBT BCIP.
Digestion products were visualized using Sybr Gold (Life Technologies S-11494).
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