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Extracellular bacteria were visualized by anti-rabbit secondary antibody conjugated to Alexa Fluor 488 (Invitrogen), whereas total bacteria were visualized by the autofluorescence of Mycobacterium as indicated below.
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Bacteria were visualized with a live/dead stain.
To identify internalized bacteria at least 10 optical sections (0.2 0.5 mm) spanning the entire DC were visualized by open source software program Fiji (http://fiji.sc/Fiji).sc/Fiji
Pedestals were visualized by immunofluorescent staining of actin using fluorescent phalloidin and bacteria with DAPI.
Liquid droplets were visualized by DIC microscopy.
Nuclei were visualized by DAPI (blue).
Both particles were visualized by TEM.
Polymer remnants were visualized by polarization microscopy.
The membrane reactions were visualized by Perkin Elmer-enhanchemiluminescenceence reagents.
Following secondary antibody incubations, signals were visualized by enhanced chemiluminescence.
Bands were visualized by enhanced chemiluminescence (Pierce Biotechnology).
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