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Bacteria were then removed by centrifugation and the supernatant sterilized by passage through a 0.22 µm cellulose acetate filter.
The culture medium and free-floating bacteria were then removed by aspiration.
Non‐adherent bacteria were then removed by washing in RPMI three times.
Culture medium and free-floating bacteria were then removed by aspiration, the wells were washed twice with PBS, and the biofilms were stained with 0.05% crystal violet dye (100 μL) for 10 min.
Similar(55)
The medium containing any nonadherent bacteria was then removed, and serial dilutions of derivatives in 200 μl fresh TSB were added to the wells and incubated for another 16 h at 37 °C.
Non ingested bacteria (Staphylococcus aureus) were then removed by differential centrifugation for 10 min at 10000 rpm at 4°C.
Their names were then removed.
Bladder catheters were then removed.
Hermaphrodites were then removed.
Redundant targets were then removed.
Nonadherent cells were then removed.
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