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Exact(8)
Bacteria were then added to treatments at 107 CFU/mL in a volume of 400 μL and incubated aerobically at 37°C for 3 hours.
104 LM-OVA bacteria were then added to the flask and the infected culture was incubated overnight at 37°C.
108 bacteria were then added into 2 ml of complete cell culture medium without antibiotics and incubated 30 min at 26°C.
After a temperature shift to 37°C, bacteria were then added to cell cultures at a calculated MOI of 10 in 12 well plates.
UV-killed bacteria were then added as 10 µL medium per well to a final concentration ranging from 103 to 107 CFU/mL, i.e. to a MOI ranging from 0.01 to 100.
The Brucella bacteria were then added to achieve a final bacterial concentration of 10 cfu/mL.
Similar(52)
Bacteria (10 MOI) were then added to the rat BMDM for 2 hr, gentamicin treated, and intracellular numbers analyzed as above.
These extracts were then added to LUG960 (recipient) cells; the treated bacteria were then tested for their capacity to adhere to collagen IV from commercial source (Sigma-Aldrich).
Antibodies were then added sequentially.
Amylase inducers were then added.
More suggestions(16)
media were then added
viruses were then added
diseases were then added
bacteria were then analyzed
bacteria were then spun
bacteria were then propagated
bacteria were then removed
bacteria were then used
bacteria were then spread
bacteria were then sorted
bacteria were then collected
bacteria were then examined
bacteria were then fixed
bacteria were then resuspended
bacteria were then calculated
bacteria were then decanted
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