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Bacteria were stored at −80°C in 15% glycerol in brain heart infusion broth.
Bacteria were stored at −80°C in 20% glycerol stocks for further experiments.
All bacteria were stored at −80°C in growth medium at ∼5×108 CFU/ml.
After incubation, bacteria were stored in glycerol stocks containing 300 µl glycerol and 700 µl medium at −80°C.
All bacteria were stored in 50% medium and 50% glycerol solution in freezers at −80°C before use.
Bacteria were stored as 30% glycerol stocks at −80°C and subcultured on blood agar plates at 37°C before testing.
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About 3,000 strains of bacteria are stored at the center, many of them in cryogenic casks cooled with liquid nitrogen and isolated from the environment by layered enclosures and oversize air-handling systems, and all dependent on electricity.
The bacterium was stored at −80°C in cation-adjusted Mueller-Hinton II (Ca-MH) broth with 10% glycerol (Sigma-Aldrich, Dorset, United Kingdom).
The bacteria suspensions were stored in 200 μl aliquots at −80°C. 100 μl of competent bacteria were mixed with 1 μl of control pUC18 plasmid [0.1 ng/μl in nuclease-free water (Agilent, 200231-42)] in cold 14 ml round bottomed tubes (BD, Product no. 352059) and incubated on ice for 30 min.
Bacteria stocks were stored at −80°C.
Following three steps of washing with saline solution to remove unbound bacteria, the pieces were stored at 4°C under sterile conditions during the transfer to the experiment laboratory and until the time of surgery.
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