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Plates were washed three times, and attached bacteria were stained using 0.1% (w/v) crystal violet.
To check whether bacteria were Gram-positive or Gram-negative, the bacteria were stained using Gram's method as described by the manufacturer (Sigma Aldrich).
Direct observation of individual bacteria on an electrode, when bacteria were stained using acridine orange, was not possible due to high levels of background fluorescence.
The internalized bacteria were stained using a rabbit anti-Shigella antibody.
Bacteria were stained using the LIVE/DEAD BacLightTM viability assay L-7012 (Molecular Probes, Invitrogen, Carlsbad, CA).
Bacteria were stained using immunofluorescence.
Similar(54)
Gels were stained using 1.5 μl/ml of ethidium bromide.
Macrophages and S. aureus bacteria were stained according to standard protocols using an appropriate dilution of a monoclonal rat anti-mouse F4/80 antibody (clone BM8, purchased from Biolegend, Fell, Germany) or a polyclonal rabbit anti-S.
Adherent bacteria were stained with Syto-9 and examined by CLSM.
Live bacteria were stained with SYTO 9 to emit a green fluorescence signal, and dead bacteria were stained with propidium iodide to produce red fluorescence.
After another 24 h of incubation, the bacteria were stained and examined as previously mentioned.
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