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For example, in one directed evolution programme, bacteria were selected on the basis of being able to utilise l-rhamnose as a sole carbon source: such bacteria were able to survive through expression of a variant aldolase able to cleave l-rhamnulose [49].
Colonies of transformed bacteria were selected on Ampicillin (50 μg/ml) containing agar plates using blue/white selection (LB-agar plates: 1% NaCl, 1% Trypton, 0.5% yeast extract, 1.5% agar, 60 μg/ml Isopropyl-β-D-thiogalactopyranosid, 40 μg/ml X-Gal).
Transformed bacteria were selected on LB-plates containing either ampicillin, or kanamycin depending on respective bacterial selection markers (Table 1).
These bacteria were selected on ampicillin-agar plate at 37°C for 16 h, then bacteria with the interest plasmid were grown in 200 mL of LB medium (Bacto-tryptone 10 g, bacto-yeast extract 5 g, NaCl 10 g) with ampicillin.
Transformed bacteria were selected on LB-plates containing ampicillin.
Recombinant bacteria were selected on LB agar plates containing 10 μg/ml of cefoxitin.
Similar(49)
Sequences from other symbionts and free-living bacteria were selected for inclusion on the basis of sequence similarity, using the BLAST search tool (NCBI).
In this study, 24 endophytic bacteria were selected for tests based on biocontrol traits such as indole-3-acetic acid and siderophore production, solubilization of phosphate, and inhibition growth of P. syringae pv.
Several uncharacterized proteins from different bacteria were selected to test for PTDH activity based on their level of sequence identity (44 56%) to wt-PTDH from P. stutzeri (Table S1 of the Supporting Information).
Fifty oral bacteria were selected based on their frequency in the dataset and presence in the Human Oral Microbiome Database32 and categorized into groups based on whether they are known to be Gram stain positive or Gram stain negative, the presence or absence of a surface layer (S-layer), and overall genomic GC content (Figs 5a and c).
The best cellulase- (VCE-19) and xylanase-producing (VXE-41) bacteria were selected based on their enzyme activities (cellulase and xylanase), their ability to ferment sugar to ethanol and their tolerance to ethanol concentration of up to 6%% (v/v).
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bacteria was selected on
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