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For plate assays, bacteria were seeded into soft LB or M9 agar (0.3% agar) with or without X-gal (40 µg/ml) as indicated in text.
Diluted overnight cultures of bacteria were seeded into wells containing serially diluted antibiotic stocks, from concentrations ranging from 0 to 1,024 µg/ml.
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Bacteria were seeded directly onto NGM plates containing 2 mM IPTG and 25 µg/ml carbenicillin.
Briefly, triplicate samples of wild type B. burgdorferi or isogenic BbCRASP-2 and BbCRASP-1 mutant [25] were seeded into 1 ml tubes at a final concentration of 5×106 bacteria per ml.
3×107 macrophages were seeded into a 150 cm2 tissue culture flask and infected at a MOI of 10 bacteria to 1 MФ in binding medium.
Roy Welland, Chris Willenken, Billy Cohen, Ron Smith, Steve Garner and Howard Weinstein (who were seeded into the quarterfinals) faced Steve Robinson, Peter Boyd, Doug Doub, Adam Wildavsky, Fred Stewart and Kit Woolsey.
Rat BMDM were seeded into 96 well plates (2×105 cells/well), allowed to adhere, and then infected (10 and 100 MOI) with subsp. novicida, LVS, subsp. holarctica and subsp. tularensis for two hr to allow phagocytosis of bacteria.
HEK293T cells (3×105) were seeded into 6-wells plates.
Subcultured 3×105 SMCs were seeded into 60 mm Petri dishes.
HCAECs were seeded into 6-well plates.
MM cells were seeded into wells.
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