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The bacteria were prepared according to the manufacturer's instructions.
The tube samples containing various bacteria were prepared as above.
Heat-killed bacteria were prepared according to Noethe and Manstein (1998).
Heat-killed bacteria were prepared according to Zuany-Amorim et al. (2002).
Selective media for isolation of fungi, actinomycetes and bacteria were prepared as described previously (Ko et al. 2010b).
Prior to SEM analysis, samples of bacteria and yeast incubated on foils with GO-Ag and untreated bacteria were prepared.
Similar(26)
Accordingly, first, a suspension of E. coli LMG bacteria was prepared.
The phosphate buffer (PBS) used to suspend the bacteria was prepared with 1.42-g Na2HPO4, 0.27-g KH2PO4, 8-g NaCl, and 0.2-g KCl per liter distilled water, and the pH was adjusted with 1-M HCl or 1-M NaOH according to experimental requirements.
An overnight culture of bacteria was prepared for RNA extraction by diluting 1∶50 in 100 ml of fresh medium with aeration by rotary shaking (250 rpm).
The inoculum containing growing bacteria was prepared as in the batch culture experiment.
Bacteria outer membrane proteins (OMPs) were prepared as described in Alteri and Mobley41.
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