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The bacteria were opsonized using fresh serum from the same donor whose blood was used to prepare neutrophils.
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For assays using PMNs, bacteria were opsonized in 5% NHS.
PMN bactericidal activity toward serum-opsonized S. aureus and lysis of PMNs following phagocytosis of serum opsonized bacteria were determined using published methods [8], [9], [23].
Bacteria were detected using anti-F.t.
Bacteria were stained using immunofluorescence.
Unlabelled opsonized E. coli bacteria were used as a particulate stimulus, the protein C ligand phorbol 12-myristate 13-acetate (PMA) was used as a high stimulus, and the chemotactic peptide N-formyl-MetLeuPhe (fMLP) subsequently was used as a low physiological stimulus.
In this case, no real bacteria were used, but Shendruk said that E. coli bacteria have been commonly used in experimental works.
Samples without bacteria were used as controls.
Special bacteria are sometimes used as part of this process.
In medicine, genetically modified bacteria are used to produce insulin.
Immature DCs from patients or donors and opsonized bacteria were co-cultured for 2 hours at 37°C and their internalization was evaluated by flow cytometry, in accordance with the manufacturer's recommendations.
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