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Different types of interaction between GFP-Lc3 structures and bacteria were observed at 5 dpi, and this is shown in more detail in Figure 4.

At the site of inoculation, BD205A,D207A bacteria were observed at levels similar to WT bacteria, but were significantly higher than those of BDel202 208 inoculated mice.

However, at 6 h and 8 h after infection, lower numbers of vacuoles containing ssaV or sseG mutant bacteria in comparison to vacuoles containing wt bacteria were observed at distances within 5 μm from the MTOC (Fig. 3).

Although some bacteria were observed at various times over the first 72 hours of culture, the population remained overwhelmingly M. pulcherrima (as judged by flow cytometry; data not shown).

Foci of bacteria were observed at and just beneath the external tissue surface and within the tonsillar crypts but no yeast cells or filaments were observed in the H&E sections.

Unlike the defect in tissue colonization observed in the tibiotarsus and ear after subcutaneous inoculation, BDel202 208 bacteria were observed at WT levels in all tissues except the heart, where the bacterial load was significantly lower than that of WT bacteria following intravenous inoculation.

Substantial non-specific kill of bacteria was observed at complement concentrations ≥ 5% that can contribute to variability in the OPA data (Fig. 1).

Although the emergence of new Gram-positive and Gram-negative bacteria was observed at each reoperation, the emergence of fungi was essentially reported at the first reoperation and only minimally at the second reoperation.

Upon intravenous injection of 50 200 colony-forming units (cfu) of wildtype M. marinum at 1 day post fertilization (dpf), aggregates of intracellular bacteria are observed at 5 days post infection (dpi) (Fig.  1A).

Bacteria were observed using a fluorescent microscope (Olympus, Tokyo, Japan) at 1,000× magnification.

At H0 and H2 after infection, internalized bacteria were observed in the cytoplasm of amoebae included in a vacuole.