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The bacteria were maintained on tryptic soy agar and grown in Lysogeny broth (Difco Laboratories, Detroit, MI, USA) overnight on shake at 150 rpm 37 °C prior to experiments.
Bacteria were maintained on nutrient agar, and the fungi were kept on Sabouroud's agar slopes.
Legionella pneumophila bacteria were maintained on solid agar plates BCYE (buffered charcoal yeast extract).
Bacteria were maintained on agar containing 5 g L−1 NaCl (Roth), 4 g L−1 K2HPO4 (Roth), 1 g L−1 KH2PO4 (Roth), 15 g L−1 Proteose Peptone No. 3 (BD), 0.5 g L−1 Starch (Sigma Aldrich), 10 ml IsoVitaleX Enrichment (BD), Bactor Agar 10 g L−1 (BD) and were grown at 37°C and 5% CO2.
The test bacteria were maintained on nutrient agar slants.
During the experiment, bacteria were maintained on blood agar plates at 8°C.
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The culture of P. acnes (anaerobic bacteria) was maintained on Anaerobic Blood Agar Medium supplemented with freshsheep blood.
The culture of aerobic bacteria was maintained on Nutrient Agar and incubated in BOD incubator for their appropriate growth (Fig. 1).
Prior to investigation a stock culture of the bacteria was maintained on LA plate.
The bacteria were maintained at 4°C on tryptic soy agar (TSA), and C. albicans (ATCC 752) was maintained on potato dextrose agar (PDA) (Difco, NJ, USA) before use.
The bacteria were maintained at 4 °C on the solid medium, and they were also stored in 15 % glycerol at −20 °C as cryoprotectant.
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