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No other pathogenic bacteria were isolated on culture.
In this study, bacteria were isolated on day 3, as the colonization and infection began [ 14, 21].
The bacteria were isolated on sheep blood agar within 20 24 h at a temperature of 37 °C in aerobic conditions.
Bacteria were isolated on Columbia agar (Oxoid, Basingstoke, UK) supplemented with 5% defibrinated sheep blood, 12 mg/l kanamycin sulphate and 30 000 IU/l polymixin B sulphate.
No infection or bacteria were isolated on day 0 (first day of injuries), as the burn wound surface is sterile immediately following thermal injury, as reported by Church and colleagues [ 21].
For each assay, bacteria were isolated on CYE plates and grown in AYE broth in 24-well plates with shaking at 37°C until bacteria were found to be highly motile (A600 > 3.2).
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Bacteria were isolated from stocks on selective BIN medium [61].
In this study, soil bacteria were isolated by enrichment on Kraft lignin and evaluated for their ligninolytic potential as a source of novel enzymes for waste lignin valorization.
Effluent samples were collected from a composite of the three sampling days, and bacteria were isolated from fermenter flasks on the last day of each period for chemical analysis.
Up to 7 different colonies of the coliform bacteria were isolated from each sample (on the blood, MacConkey and deoxycholate agars).
Twenty-five bacteria were isolated from the four stations on ASW medium supplemented with crude oil as the sole carbon source.
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