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These bacteria were inoculated into ciliate vessel, to maintain protozoan life, before doing experiments.
Into a 96-well plate uniformly filled with rich media (THB), S. mutans bacteria were inoculated into alternating wells using an automated liquid handling robot (a).
Here, biofilms formed spontaneously when planktonic S. mutans bacteria were inoculated in BFM (with decreased nutrients and excess sugar), secreting significant EPS and becoming adherent.
All bacteria were inoculated into lysogeny broth (LB) and grown for 6 h at 37°C with shaking at 165 rpm.
(vi) For the optimization of inoculum dose, the bacteria were inoculated into the screening medium (pH 7.0) containing 1 g/L of NH4 +-N, followed by incubation at 30 °C and 120 rpm for 48 h.
Overnight cultured bacteria were inoculated into 5 ml of liquid LB culture media at the ratio of 1 100, in the presence of control medium, 25 µg/ml ampicillin, or indicated amount of GFP-2 culture media, and cultured at 37 °C with shaking at 220 rpm.
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Briefly, a 1% volume of pre-cultured solutions (stationary-phase bacteria) was inoculated into new culture medium and incubated until the cells reached the late-stationary phase.
The cost of motility should therefore be much higher in HDI treatment, where bacteria are inoculated at high density, than in LDI condition, where density is initially low.
The bacteria was inoculated into this medium at 1 × 10 CFU/ml concentration based on optical density (O.D).
When bacteria are inoculated into fresh medium, they often show a period without growth known as the lag phase [ 1- 3].
For these assays, flies are either fed different strains of bacteria obtained from burn wounds or from cystic fibrosis patients, or bacteria are inoculated by wounding flies.
More suggestions(14)
cells were inoculated
bacteria were applied
strains were inoculated
bacteria were performed
media were inoculated
viruses were inoculated
organisms were inoculated
bacteria were seeded
cultures were inoculated
bacteria were injected
bacteria were cultured
bacteria were spiked
bacteria were were
microorganisms were inoculated
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