The bacteria were grown on a nutrient agar surface at 7 °C.
Bacteria were grown on TM plates for 48 h at 36 °C in a growth chamber containing 5% CO2.
Bacteria were grown on culture plates of Trypticase Soy Agar (TSA, Scharlau) medium and were incubated at 28 °C for 24 h.
Next, they added a selection pressure: The bacteria were grown on food lacking the amino acids that CM helps to make.
For Xoo inoculation, the bacteria were grown on peptone-sucrose-agar plates at 28 °C for 2 days and resuspended in 10 mM MgCl2 to an OD600 of 0.5 (Hwang et al. 2011).
The bacteria were grown on a sulfur-free culture medium (bifidus selective medium (BSM) composed of 2.44 g KH2PO4, 5.47 g Na2HPO4, 0.2 g MgCl2.6H2O, 0.001 g CaCl2.2H2O, 0.001 g FeCl3.6H2O, 0.004 g MnCl2.4H2O, and 2 mL glycerol in 1 L deionized water.
The bacteria were grown on standard medium under recommended conditions.
Therefore, the bacteria were grown on Glc-CDMA for subsequent extraction of CLC.
Briefly, bacteria were grown on LB agar plates for 72 hours at 37°C.
The bacteria were grown on LB agar plates or in LB medium.
For scanning electron microscopy, tissue cultures infected with the same bacteria were grown on coverslips and treated as described above.
