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Lyme bacteria were grown from skin or blood samples taken from the patients when they had the rash, and the researchers analyzed a bacterial gene that varies from one strain to another.
Bacteria were grown from the single colony aerobically in 1000 ml flasks containing MOPS minimal medium [67] (obtained from Teknova CA, product number M2006) in 37°C.
As a positive control, the ΔospC/FL/1 bacteria were grown from all of the remote tissues but the ear at first week; all sites became culture positive at 2 weeks after initial inoculation (Table 6).
As a positive control, the ΔospC/FL/1 bacteria were grown from all of the skin, joint and heart specimens but from none of the ear samples at first week; all sites became culture positive at 2 weeks after initial inoculation (Table 5).
As a positive control, the ΔdbpAB/dbpAB/1 and ΔdbpAB/dbpAB/2 bacteria were grown from 6 joint, 5 remote skin, and 2 heart specimens of 6 examined mice at 1 week; all sites became culture-positive at 2 weeks after inoculation (Table 2), consistent with our previous study that showed the parental clone 13A carrying pBBE22 colonized these tissues within 2 weeks [22].
The ΔdbpAB/dbpA'/1 and ΔdbpAB/dbpA'/2 bacteria were grown from 6 joint, 4 remote skin and 2 heart specimens of 6 examined mice at 1 week, and from 6 joint, 5 remote skin and 3 heart specimens of 6 mice at 2 weeks; all sites became culture-positive at 3 weeks.
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Bacteria were grown as follows: fresh colonies from a TSA blood-agar plate were picked to inoculate 15 ml of LB medium, MGM, and MGM+0.8% sodium bicarbonate, and grown at 37°C with normal aeration or with 10% CO2 for bicarbonate cultures, with shaking at 200 rpm to mid log phase (OD600∼0.4 0.5).
From these stocks, bacteria were grown on Sabouraud agar at 30°C for 4 to 7 days; then a single colony was placed in a 7 mL sterile Eveljham-Potter device.
In an attempt to mimic their co-existence and growth as observed from field outbreaks, bacteria were grown in tubings in vitro, either in mono-culture or in co-culture.
Bacteria were grown, as previously described [5] [7].
The bacteria were grown on Columbia plates.
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