Sentence examples for bacteria were fixed using from inspiring English sources

At selected times, bacteria were fixed using 2% (w/v) paraformaldehyde in PBS for 10 min at room temperature, resuspended in PBS solution and kept at 4 °C until analysis by flow cytometry.

For scanning electron microscopy of H. pylori, the bacteria were fixed using 0.1% poly-L-lysine and dehydrated in ethanol and ethanol: isoamyl acetate solution.

The specimens with remaining attached bacteria were fixed using 0.25 mL of 2.5% glutaraldehyde per well for 15 min and, subsequently, air-dried.

HL-60 cells (105 cfu/well) were added to opsonized bacteria in a microtiter plate and incubated for 30 minutes at 37°C with shaking, after which the bacteria and cells were fixed using 3% paraformaldehyde and analyzed using a Cytomics FC500 Beckman Coulter flow cytometer (Beckman Coulter, Miami, USA).

Cells were fixed using Cytofix Cell Fixative BD Biosciencess).

The heads were fixed using different fixing agents (Table  1).

Tissues were fixed using formalin solution.

Embryos were fixed using standard protocols.

Bacteria were fixed in 2% paraformaldehyde and surface-bound IgG or IgA was detected using anti-mouse IgG-F c or IgA α-chain-FITC-conjugated antibodies (Sigma-Aldrich) on a BD LSRII flow cytometer (Biosciences, San Jose, CA, USA).

To study DNA morphology of bacteria or cells, treated bacteria were fixed in 4% paraformaldehyde and exposed to 300 nM DAPI (Invitrogen) and viewed by fluorescence microscopy using a Leica DMI6000 microscope (Leica Microsystems, Bannockburn, IL).

The slides were air-dried and the bacteria were fixed by heat.