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Thereafter, bacteria were collected by centrifugation, suspended in 0.9 % NaCl and sonicated for 5 s at 40%% amplitude for posterior CFU counting.
The bacteria were collected by centrifugation at 10,000× g for 5 min at 4°C and then washed three times with pre-chilled PBS.
The bacteria were collected by adding sterilized ultrapure water and the concentration of bacteria was adjusted to 0.3 OD at 600 nm.
The bacteria were collected by centrifugation at 100×g for 10 min, washed twice with 0.85% NaCl and re-suspended in 10 mL of 0.85% NaCl.
Then, the bacteria were collected by centrifugation at 10,000× g for 5 min at 4°C, followed by wash for three times with pre-chilled phosphate-buffered saline (PBS; 10 mmol/l, pH 7.4).
Bacteria were collected by centrifugation and adjusted to an OD600 of 0.3.
Similar(18)
Bacteria were collected the following day by centrifugation.
The intact Salmonella Typhi bacteria were collected from the specimen by centrifugation and the DNA extracted with QIAamp DNA mini kit.
After an overnight incubation at 30°C the bacteria were collected and the phagemids rescued by the addition of M13KO7 helper phage (helper phage:bacteria = 20 1).
The cecal wall was then washed 3 times in PBS with gentle shaking to remove all luminal bacteria and mucus associated bacteria were collected from the washed cecum by scraping the mucus with a plastic scapula.
Bacteria were collected at 3, 5, 7, and 9 h, and then the bacteria were cracked by lysozyme.
More suggestions(16)
organizations were collected by
cultures were collected by
cells were collected by
strains were collected by
viruses were collected by
microorganisms were collected by
bacteria were controlled by
bacteria were permeabilized by
bacteria were separated by
bacteria were phagocytosed by
bacteria were evinced by
bacteria were developed by
bacteria were inhibited by
bacteria were investigated by
bacteria were identified by
bacteria were affected by
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CEO of Professional Science Editing for Scientists @ prosciediting.com