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Correlations between environmental variables (iron, manganese, ammonia, DO, temperature and layer height) and functional microorganisms (16 characteristic bacteria) were assessed, using canonical correspondence analysis (CCA).
The streptococcus thermophiles activity of the bacteria were assessed using the Tween 80 hydrolysis test that was performed as previously reported [ 24].
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The intracellular location of bacteria was assessed using Z-stack CLSM analysis.
The survival of the bacteria was assessed using PCR-based detection of P. acidilactici MTCC5101 in fecal samples.
The association between periodontitis (BOP positive or PPD ≥ 4 mm) and detection of bacteria was assessed using multiple logistic regression analyses adjusting for age, gender, oral hygiene and smoking status.
Significance of pre- to post-intervention reductions in bacteria levels within groups were assessed using paired T-tests.
Time trends were assessed using birth cohorts.
Potential interactions were assessed using logistic regression.
Differences between groups were assessed using ANOVA.
Drug antagonism of bacteria growth was assessed using the equation: A b c o n t r o l − A b d r u g A b c o n t r o l × 100 where Abcontrol and Abdrug are the maximum spectroscopic absorbance at 600λ measured within 16 hr of bacterial growth in untreated and drug treated conditions, respectively.
Finally, as suggested by our recent refinements of the PCR test, the viable bacteria load may be assessed using a reverse-transcription based PCR protocol that targets viability-sensitive bacterial mRNAs [14] and 16S rRNA [13].
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