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The counts of surviving bacteria were analyzed using the log rank test.

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In Fig.  3c, a biofilm sample containing anammox bacteria is analyzed using parental ion scanning of the PC headgroup.

The mean quantities of biofilms formed on Days 5, 7, and 9 for each bacterium were analyzed using repeated-measures ANOVA followed by the Bonferroni correction to investigate whether the BF changed among these 3 days.

The interaction of AgNPs with bacterium was analyzed using TEM micrographs.

The eight remaining organisms (including two bacteria and six archaea), were analyzed using MetaGeneAnnotator [ 57] to identify genes and MetaTISA [ 22] to locate Translation Initiation Sites (TISs).

Twenty seven different bacteria isolates from 12 species were analyzed using intrinsic surface-enhanced Raman scattering (SERS) spectra with recently developed vancomycin coated silver nanorod (VAN AgNR) substrates.

Various physico-chemical parameters such as temperature, pH, EC, TDS, COD and chlorophyll a were analyzed using APHA standard methods Water was also monitored for heterotrophic bacteria, total coliforms, Escherichia coli and antibiotic resistant bacteria.

Images were analyzed using Gap Light Analyzer41.

Differentially expressed genes were analyzed using edgeR54.

Data were analyzed using GraphPad Prism version 6.0 (RRID:SCR_015807).

Data were analyzed using one-way ANOVA.

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