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The bacteria were analyzed by targeting the 16S rRNA gene.
Both culturable and DNA counts of the collected bacteria were analyzed to determine the final bacterial concentration.
Besides, the motilities of these bacteria were analyzed by microscopic tracking.
Ten different panels PM11-PM200) that enable chemical sensitivity tests for bacteria, were analyzed.
Fluorescent bacteria were analyzed on a FACS Caliber (BD Biosciences) flow cytometer.
Bacteria (108/ml, 100 µl) or mitochondria (3 mg/ml protein concentration, 25 µl) were incubated with fluorescently labelled HAMLET at 37°C for various 30 min. The fluorescence intensity of the bacteria were analyzed in a FACSCalibur flow cytometer (BD) using a 520 nm band-pass filter or bacteria and mitochondria were counterstained with 300 nM DAPI and inspected by confocal microscopy.
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The biodiversity of isolated bacteria was analyzed by amplified ribosomal DNA restriction analysis (ARDRA) using four restriction enzymes.
Selection in the evolution of carotenoid biosynthesis in the purple bacteria is analyzed in greater detail elsewhere [75] and therefore is considered only briefly here.
The spatial distribution of bacteria was analyzed by complete dissection of surface/muscle tissue, gut wall, and gut content of all eight longitudinal segments.
In Fig. 3c, a biofilm sample containing anammox bacteria is analyzed using parental ion scanning of the PC headgroup.
However, when the effect of the number of internalized bacteria was analyzed, MOI 2.5 and MOI 40 were used as well.
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