If an equal number of typhoid bacteria are spread on a person's skin and on a glass plate, those on the skin die much more quickly than do those on the plate, suggesting that the skin has some bactericidal property against typhoid germs.
Certain amounts of different fungi and bacteria were spread on naturally aged paper.
Bacteria are spread on the surface of an agar plate and then the strip is placed on the plate.
The transformed bacteria were spread on a LB plate containing amplicilin, IPTG and X-gal before overnight incubation.
Bacteria were spread on selective media (LB-agar plates, 100 μg/ml ampicillin, 10 μg/ml tetracycline and 1% (w/v) glucose) and grown over night at 37°C.
Aliquots of 50 μl and 200 μl of transformed bacteria were spread on 2xYT plates containing 100 μg/ml ampicillin and incubated overnight at 37°C.
Recipient bacteria were spread on LB agar containing hygromycin B. After overnight incubation at 37°C, 50 colonies were selected at random, plasmid DNA was harvested, and genomic DNA inserts were sequenced using primer TN5-9R (Table 2).
For BAC linking, about 1.5 μg of I- PpoI digested BAC DNA (not purified) was electroporated and the bacteria were spread on plates containing 5 μg/ml gentamycin.
After electroporation as described above, the bacteria were spread on plates containing 50 μg/ml of spectinomycin or 5 μg/ml gentamycin respectively.
To isolate cadmium resistant bacteria, 100 µL of wastewater sample was spread on Luria-Bertani (LB) agar (1 % NaCl, 1 % tryptone, 0.5 % yeast extract and 1.5 % agar) plates supplemented with 1 mM Cd2+.
