For example, in one directed evolution programme, bacteria were selected on the basis of being able to utilise l-rhamnose as a sole carbon source: such bacteria were able to survive through expression of a variant aldolase able to cleave l-rhamnulose [49].
Colonies of transformed bacteria were selected on Ampicillin (50 μg/ml) containing agar plates using blue/white selection (LB-agar plates: 1% NaCl, 1% Trypton, 0.5% yeast extract, 1.5% agar, 60 μg/ml Isopropyl-β-D-thiogalactopyranosid, 40 μg/ml X-Gal).
Transformed bacteria were selected on LB-plates containing either ampicillin, or kanamycin depending on respective bacterial selection markers (Table 1).
These bacteria were selected on ampicillin-agar plate at 37°C for 16 h, then bacteria with the interest plasmid were grown in 200 mL of LB medium (Bacto-tryptone 10 g, bacto-yeast extract 5 g, NaCl 10 g) with ampicillin.
Transformed bacteria were selected on LB-plates containing ampicillin.
Recombinant bacteria were selected on LB agar plates containing 10 μg/ml of cefoxitin.
The transformant bacteria were selected on LB agar containing ampicillin (100 μg/ml).
Following verification, the mutants were P22-transduced into a clean SL1344 background and non-lysogenic bacteria were selected on Green Agar plates.
Resulting plasmids 35S DIR1-EYFP, 35S DIR1-EYFP5-EYFP and 35S:EYFP were sequenceDIR1Δ1-25-EYFP DIR1Δ1-25-EYFP DIR1Δ1-25-EYFPstrand GV35S EYFP90 by electroporation and transformed bacteria were sequencedon 2YT mobilizedntaintog rifAgrobacteriumamycin and spectumefaciens
The vector contains an ampicillin (Amp) resistance and strain HT115 is tetracycline (Tet) resistant, so bacteria were selected on Amp (75 μg/ml) and Tet (12.5 μg/ml) plates.
The constructed pRM-vgb vector was electro-transformed into A. tumefaciens LBA4404, and recombinant bacteria were selected on LB agar plates containing 100 μg/ml streptomycin and 50 μg/ml kanamycin.
