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Chromosomal DNA of screened bacteria was extracted using CinnaPure TM DNA extraction kit (Cinnagen, Iran) according to manufacturer's instructions.
Genomic DNA of isolated bacteria was extracted using the DNeasy® 96 Blood and Tissue Kit (Qiagen).
Genomic DNA of bacteria was extracted using the QIAamp DNA purification kit (Qiagen) according to the manufacturer's instructions.
DNA from stored progeny bacteria was extracted using the PureElute Bacterial Genomic DNA preparation kit (EdgeBiosystems, UK) following the standard protocol with the addition of lysostaphin (Lindsay et al. 2006).
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The differences in M1 protein were also observed in mutanolysin/lysozyme extracts, as well as when bacteria were extracted using phage lysin, PlyC [39] (data not shown).
Chromosomal and plasmid DNA of the MSSA and MRSA bacteria were extracted using Wizard® Genomic DNA Purification Kit with accessory reagents (Promega, USA); the method of extraction was done according to the manufacturer's instructions.
The paper point was transferred into 200 μl of phosphate-buffered saline (PBS) and centrifuged at 12,000 × g for 5 min. After centrifugation, the bacteria chromosomal DNA was extracted using MORA-extract (Cosmo Bio, Tokyo, Japan), according to the manufacturer's instructions, and then served as a template.
Genomic DNA of A.baumannii isolates was extracted using TIANamp Bacteria DNA Kit(Tiangen).
Total bacteria DNA from the clinical samples was extracted using the QIAamp DNA Mini Kit (Qiagen, Germany).
Total RNA was extracted using RiboPure-Bacteria kit (Ambion).
Genomic DNA was extracted using the TIANamp bacteria DNA kit (Tiangen Biotech (Beijing) Co., Ltd).
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