Finally are the so-called sulphophilic fauna that consist of a diverse collection of bone-feeders, grazers of bacteria, suspension and deposit feeders, and various predators drawn to the action.
Then 100 μL of the above PBS diluted bacteria suspension was then placed onto the surface of samples.
The negative control was taken as LB broth bacteria suspension without any agent and blank as only medium and positive control as medium with agents.
The compounds were dissolved in DMSO and then added to bacteria suspension to obtain the final concentration of 5%% (v/v) DMSO or less.
The blank (150 µL broth) and the bacteria (100 µL broth and 50 µL bacteria suspension) were prepared and gentamicin and ciprofloxacin were used as positive controls.
After blotting off the excess bacteria suspension, leaf discs were transferred to MS basal medium containing 100 μM acetosyringone and co-cultivated for 48 h.
Each experiment was repeated three times and Norfloxacin, bacteria suspension of 5%% (v/v) DMSO were used as a positive control and a blank control, respectively.
Then 10 μl each of bacteria suspension was dispersed on the culture medium and incubated for 24 h at 37 °C.
One milliliter of the bacteria suspension was added to 100 ml of previously autoclaved, warm Mueller Hinton agar (MHA)/Sabouraud dextrose agar.
These findings are beneficial to enhance the performance of the bioreactor by the optimal design of aeration system for bioconversion of methane fed into methane oxidizing bacteria suspension.
Also, in free bacteria sample, 1 ml of free bacteria suspension harvested from simulated intestinal juice was used for cell count by "pour plate count method" (see "Enumeration of free and microencapsulated probiotic bacteria").
