Control plates had the bacteria seeded on the cannula entry point.
Growing C. elegans on lawns of dividing bacteria seeded at differing concentrations [17] may well result in reduction of both food intake and also bacterial invasion and as such at least part of the life enhancing mechanism invoked by this DR protocol may be specific to C. elegans.
RNAi was performed via feeding as described using HT115 DE3) bacteria seeded on MYOB plates containing 2 mM IPTG and 25 μg/mL carbenicillin (Timmons and Fire 1998).
Effects of released curcumin were comparable with those observed from the inhibition study with bacteria seeded directly on curcumin-loaded micelle-LBL networks, confirming the antibacterial role played by curcumin.
Our study presents the following limitations: it is a preliminary evaluation and focuses on the in vitro effect of a minimal dose of ozone applied in Petri dishes containing bacteria seeded superficially on the Agar medium.
unc-22 bacteria were then serially diluted with the empty vector bacteria and seeded onto NGM agar plates containing IPTG (1 mM) and carbenicillin (25 μg/ml).
Bacteria were seeded in a mineral medium containing 0.5% carboxymethylcellulose as sole carbon source for detection of cellulose activity.
Bacteria were seeded directly onto NGM plates containing 2 mM IPTG and 25 µg/ml carbenicillin.
For plate assays, bacteria were seeded into soft LB or M9 agar (0.3% agar) with or without X-gal (40 µg/ml) as indicated in text.
The bacteria were seeded with a confluent NIH 3T3 (mouse fibroblast) cell monolayer overnight for biofilm formation.
