Sentence examples for bacteria procedure from inspiring English sources

Briefly, RNA was extracted using RNeasy mini kit (Qiagen, Carlsbad CA), and samples obtained from three different cultures were pooled and amplified following the MessageAmp II Bacteria procedure (Ambion, Austin TX). 1 μg of RNA was polyadenylated and the complementary strand was synthesized by reverse transcription primed with T7-oligo-dT.

Cultures were incubated for 2 h, and two (22 ml) aliquots were taken from each and prepared as described to remove bacteria (Adaptation Procedure).

This approach combines a simple bacteria lysis procedure with on-chip LAMP detection, without using any centrifuge and prepurified DNA templates.

In all cases, a bacterium-enrichment procedure was undertaken by selecting particular cell types/tissues and/or by adopting specific experimental procedures (selective hybridization, host rRNA depletion or polyA transcript depletion).

Organic farmers compost the manure to kill the bacteria, but the procedure is not always followed properly, especially by uncertified farmers.

Although the immunological method, like enzyme-linked immunosorbent assay (ELISA), is a widely used method for bacteria detection, the procedure is complex and time-consuming.

Genetic manipulation of bacteria is a procedure necessary to obtain new strains that express peculiar and defined genetic determinants or to introduce genetic variants responsible for phenotypic modifications.

In addition, extensively exposed submucosa, especially with an ESD, directly contacts colonic luminal bacteria after the procedure, which may increase the risk of bacteremia.

We extracted total DNA within one week of collection using the Puregene Bacteria DNA purification procedure with the following modifications.

A mock homogenisation and extraction without sample material was included in each extraction run to exclude contamination with bacteria during the procedure.

Evaluation of the bacteriological cultures of the ETT lavage with saline after extubation of mechanically ventilated patients to verify dislocation of bacteria through this procedure.