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Quantitative PCR was performed to analyze the total number of bacteria present in each sample.
The number of bacteria present in each BMDC section ranged from 1 to 24 with an average of 7 bacteria per cell.
The total number of bacteria present in each tracheal sample was also analysed against its mucin content showing that the samples with increased amounts Muc5b and Muc5ac also had higher total bacterial counts (Figure 8, p<0.001)).
In addition, the types and number of bacteria present in each tracheal wash had been determined immediately after return of samples on ice in a cool box to the laboratory.
For positive samples only, we tested whether vaccination had an effect on the relative amount of bacteria present in each sample type, as indicated by the Ct value.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDITOF-MS) was used to identify the bacteria present in each specimen.
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When the level of drosomycin expression was normalized for the number of bacteria present in infected flies at each particular time point, the activation of the drosomycin expression was higher for the atl mutant than for the wild type S. aureus strain at 12 h post-infection.
Colony forming units (CFU) were counted on each plate to estimate the number of Db11 bacteria present in the whole fly.
Were the bacteria present in living patients?
The bacteria present in yoghurt create lactic acid, which acts as the fermenter here.
Many scientific studies have confirmed that they have an especially full flavor thanks to the ripening activity of harmless bacteria present in the milk.
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