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We attribute the initial decrease of bacteria in solution to the lack of substrate (the treatments were prepared in DI water) required to support bacterial growth during the early stages of the experiment.
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Figure 3 Cell density plots for (a) bacteria bound to lipid/pyrite, (b) bacteria in solution for lipid/pyrite as a function of time.
We have developed hyperbranched poly NIPAM) polymers functionalized with the antibiotics Vancomycin and Polymyxin-B that are sensitive to the presence of bacteria in solution.
Simple shaking of the plates for 25 min on a microtiter plate shaker prior to each OD600 measurement was found to be sufficient to dissolve bacterial aggregates and resuspend the bacteria in solution.
This means that bacteria in solution experience only a repulsive force, and thus they cannot approach to the sand surface.
In addition, due to the bactericidal property of silver, this autonomous micromotor is capable of killing bacteria in solution.
The particles were subsequently washed so that any bacteria in solution would pass through the filter.
The concentration of bacteria in solution was verified retrospectively by plating and culture.
From the captured bacteria in solution on the dielectrophoretic chip, high-quality Raman spectra have been recorded within 1 second.
For a better understanding of a disinfectant's effectiveness and standardization of use in hospital sanitation programs, the tested bacteria in solution were considered standard biological indicators (BI).
The researchers performed both preincubation of bacteria in solution with XF73 followed by subsequent application on the ex vivo porcine skin and application of bacteria on the skin followed by an incubation with XF73.
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