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What is more, they are much faster than traditional methods and allow qualitative and (semi) quantitative detection of bacteria in complex environments.
In our opinion, the presented multiplex PCR assay will be very useful to monitor the presence of pectinolytic bacteria in complex environments.
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For bacteria living in complex environments it may be assumed that there would be a pressure to continually expand the metabolic and functional flexibility and therefore the genome size.
Antimicrobial peptides are produced as defensin molecules by many Gram-positive bacteria including Bacillus in complex environments.
The need for training in complex environments is often underestimated.
Since periodontal tissues are constantly exposed to a variety of microbial pathogens the innate immune response has a primary role in the defense against plaque-associated bacteria in this complex environment [ 2, 3].
This may indicate that GC rich microbes live in more complex environments than intracellular bacteria [ 20].
Bacteria that inhabit stable environments (extremophiles, obligate parasites or symbionts), which generally have small genomes, possess fewer sensory and regulatory genes than free-living bacteria found in complex and changing environments such as those living in soil or in association with plants [2].
It is presumed that a key function of the S-layer is to form a selective porosity barrier protecting the bacterium from a variety of predatorial assaults in the complex environments of bacterial biofilms.
Thus, amperometry has limited utility in chemically complex environments.
Comparative genomics and comparative network biology are emerging as key tools in understanding of how bacteria respond cooperatively to challenging complex environments.
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