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Moreover, SiCYS, SiCYS-N or SiCYS-C had no effect on the proliferation of various bacteria (data not shown).
The microscopic examination of Gram's stained culture preparation of DAB-1Y as rods in chains and DAB-1W as small cocci bacteria (data not shown).
Higher concentrations of EDTA did not further inhibit the uptake of bacteria (data not shown).
Recombinant polyhistidine-tagged Bicaudal and Stubarista were expressed and purified from bacteria (data not shown).
Similar results were obtained after inoculation with higher doses of bacteria (data not shown).
This set of experiments revealed that ∼75% of YFP+ cells harboured bacteria (data not shown).
No activity of HT61 was observed against either log-phase or stationary phase Gram negative bacteria (data not shown).
Furthermore, cell monolayers remained intact and the morphology of the cells was not affected by the bacteria (data not shown).
In addition, CD3+ T lymphocytes did not produce cytokines when co-cultured with killed bacteria (data not shown).
Of note, cell monolayers remained intact and the morphology of the cells was not affected by the bacteria (data not shown).
As expected, we were unable to detect any of the 200 OT-I cells in mice immunized with Wt bacteria (data not shown).
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