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For the subcutaneous bleomycin-induced skin and lung fibrosis model, mice backs were shaved and bleomycin administered subcutaneously daily for over 2 weeks.
Male adult rats were anaesthetized (ketamine, 60 mg/kg, and medetomidine, 0.25 mg/kg), the cervical region of their backs were shaved and cleansed with iodine and core temperature was maintained close to 37 °C using a self-regulating heated blanket.
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After anaesthesia (chloral hydrate, 400 mg/kg, i.p). the limbs and the back were shaved and tattooed in order to locate the bony processes as previously described in details [15].
Briefly, after anesthesia (intraperitoneal chloral hydrate, 400 mg/kg) the four limbs and the back were shaved and tattooed in order to locate the bony processes.
The back is shaved, leaving a rat tail at the bottom.
Mice were sacrificed at P21, and the back was shaved and treated with hair remover.
Under anesthesia, the recipient's back was shaved by clippers and disinfected by beta-iodine solution.
After the animal was anesthetized, its back was shaved and epilated with hair remover lotion (Nair, Church & Dwight Co, Princeton, NJ).
The animal's back was shaved, disinfected (betadine, 5%) and a midline incision was made over the C6-T13 sprocessesocesses.
Rats' hair of the back was shaved and the skin was cleaned with 10% povidone-iodine solution.
While anesthetized, the upper dorsum of the back was shaved with an electric razor, leaving a strip of hair in midline to define the right and left sides.
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