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Intra-cellular localization of the fluorescent reporter tGPH in fat body cells under different treatments and genetic backgrounds was scored by involving two people where one person performed the staining and the other read the slides, which have been numerically "blinded" by the first person.
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Any line that did not have a strong effect on its own but lead to a further loss of macro chaetae in a VAP over-expression background was scored as an enhancer (average macro chaetae <4.0, p<4.0,).
In addition, the percentage of purple pigmentation covering the root surface, which estimates the root total pigment content in the 70349 background, was scored visually and recorded in 70349 (from here on referred to as root "RTPE" for "root total pigment estimate").
Progeny containing CyO, P{ry[+t7.2] = sevRas85D V12 }FK1 and either heterozygous mutant candidate genes or wild-type (w 1118 ) background were scored for rough eye phenotypes.
Values of Δ less than or equal to 0 on a scale, indicate that background pain intensity was scored higher or equal to peak pain intensity with that scale (inconsistent evaluation).
The latter nodule was scored as below-background on OSEM, and background on BPL.
All experiments were performed on littermate mice with the potential to inherit each genotype and any residual mixed genetic backgrounds (most experiments were in a largely Bl6 background) and were scored by investigators blinded to the genotypes.
Cells with fluorescence intensities above background levels were scored as expressing αSMA.
Staining intensities above background levels were scored as positive.
Cells in which the dsRED signal did not exceed background levels were scored as 'miRNA positive'.
As somatic mutations may be missed due to normal tissue contamination, only sequence traces with no background signal were scored successful to enable detection of even small mutation peaks.
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