The success of such a procedure will depend on the genetic background of the respective cancer cells.
Within each fragment, amplitudes of peaks were estimated by summing the values (log2 signal vs. background) of the respective probes.
The RTK pathway intersection the authors observed in MEPM cells could easily be thoroughly validated in the background of the respective mutants.
Cell context-dependent differences exist in the mechanisms of cell death, which may be related to the genetic background of the respective cell lines, such as the differences in p53 status, but cell death was observed in either case.
In addition, a clinical evaluation of atherosclerotic patients that is not based on the presence of anti-chlamydial antibodies but on the genetic background of the respective clinical isolate could foster our understanding of the pathophysiological relevance of Cpn infections in these patients and help to optimize diagnostic tools for the identification of vascular infections.
Although the pathophysiological background of the respective mutations has to be further investigated, amplicon deep-sequencing assays may identify new prognostic parameters and contribute to more diversified therapeutic concepts, for example, by investigation of compounds targeting the RAS pathway in subsets of myeloma patients.
In contrast to traditional research in philosophical ethics, additional issues should be considered here which pertain to pragmatic aspects of conducting the research, as well as to more theoretical facets, such as the interrelation between the ethical-theoretical and the socio-theoretical background of the respective study.
Overall, genetic, cellular, and biochemical data indicate that most ciliopathies rather represent a continuum in which the phenotypic outcome is determined by the nature of the mutation in conjunction with the genetic background of the respective patient and oligogenic inheritance patterns.
Background information of the respective strains examined is described in the supplementary Table S1.
The GFP fluorescence in medium was obtained by subtraction of the background fluorescence of the respective samples from GFP-negative Jurkat cultures.
