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We observed similar phenotypes with all GAL4 drivers in both wild type and lam mutant backgrounds (data not shown).
Notably, Lfng protein was easily detected in CD4+ T cells under all assay conditions as well as naïve CD4+ T cells from different genetic backgrounds (data not shown).
Resistance was also diminished by the Δfur, furR3S and furH99 alleles and enhanced by the furR3I and furT41A alleles in these strain backgrounds (data not shown).
Locomotor activity decreased over time [F 5,265) = 33.32, p<0.0001] and this was similar in Fmr1-KO and WT of both backgrounds (data not shown).
No differences in the expression of the Villin-Cre recombinase or recombination efficiency were found between the intestines of VC+ mice in a mixed FVB/C57BL/6 and the CB57BL/6 backgrounds (data not shown).
Transient transfection of minigene constructs in HEK 293 cells was used for genomic and small molecule compound high-throughput screens (HTS) described below, due to superior luciferase signal when compared to stable clones in either HEK 293 or C2C12 cellular backgrounds (data not shown).
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The data also repeated with all Vpr mutants created in the Vpr-2 genetic background (data not shown).
In addition to this, the p40_41A construct showed little activity in an rpoS background (data not shown).
Comparable results were found for heterozygous mutant and wild-type mice of the 129X1 background (data not shown).
Uninfected RBCs always exhibited single clusters (Fig. 8A) with occasional tails that converged to the fluorescence background (data not shown).
The array data showed a significantly higher accumulation of H4K16ac along Jarid1c gene body compared to Jarid1d, the latter showing signals no higher than background (data not shown).
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