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The distributions for the backgrounds are obtained from simulation with the exception of the combinatorial background.
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Net activities from distributions in various backgrounds were obtained using a large region of interest with background subtraction.
P53+/− heterozygotes on both 129/Sv and C57BL/6 backgrounds were obtained from Karlyne Reilly of NCI-Frederick [39].
Mtm1-deficient mice in the 129Pas genetic background were obtained from Dr. J. Laporte (IGBMC, Illkirch, France).
Zbtb46-GFP (B6.129S(C -Zbtb46tm1.1Kmm/J), IRF8−/− (B6(C -Zbtb46tm1.1Kmm/Jand Batf3−/− (B6.129S(C)-Batf3tm1Kmm/J) mIRF8−/ the C57BL/6 background were obtained from The Jackson Laboratory and have been previously described18,29,66.
Targeted ES cells with C57BL/6N background were obtained by homologous recombination with a vector containing the Ap4 exons 2 4 flanked by loxP sites and an intronic neomycin resistance (Neo) cassette flanked by frt sites (scheme in Jackstadt et al.6).6
In the first cycle the oxidation peaks of copper were observed but in the second and next cycles, they were omitted and a clean background was obtained.
Dongjin background was obtained from Postech (http://www.postech.ac.kr/life/pfg/risd).ac.kr/life/pfg/risd
The background was obtained by measuring the fluorescence intensity of regions out of the cells.
Destaining of the gel was performed in a 5%methanol/7%% acetic acid/H2O solution until the desired background was obtained.
A number of transgenic plants that may have mutations in target 1/2 in Nipponbare background were obtained.
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