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Zero counts (and missing values due to high background) were substituted with the minimum observed level for each marker in lieu of a limit of quantification (LoQ).
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For oocyte batches showing high background currents at pH≤6.5, NaCl and KCl were substituted with equimolar amounts of Na- or K-gluconate.
For oocyte batches exhibiting high background chloride currents at pH⩽6.5, NaCl and KCl were substituted with equimolar amounts of Na- or K-gluconate.
Rejected signals were substituted with signals obtained via spline-interpolation73.
All corresponding missing p-values were substituted with 1.
Interestingly, when the twin arginines in ssDmsA were substituted with lysines, the BiFC signal following co-expression of TatB-Y2 and TatC-Y2 increased to 5.4- and 10.7-fold above background, respectively (Fig. 6a).
Missing data were substituted with mean values of missing items.
These positions were substituted with either alanine or cysteine.
Missing values at evaluation visits were substituted with basal values.
Tenofovir was substituted with zidovudine in six of these cases and the other 15 cases were substituted with abacavir.
When the background gas N2 was substituted with CO2, the combustion of all fuels was distinctly less intense; at moderate O2 mole fractions (<30%) most particles did not ignite under active flow conditions in the furnace (they did ignite under quiescent gas flow conditions in the DTF).
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