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Measurements with signal intensities lower than the background were set to zero.
Any corrected signals falling below the cut-off threshold (typically, fluorescence values less than twice the local background) were set to zero, giving final corrected signals for each fluorochrome for each spot.
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Brownlee's strong commercial background is set to signal a change of direction at the AOP, which has become increasingly influential since it was established in 2002.
During this process, the flame portion of the image is set to white (binary value 1) and the background is set to black (binary 0).
Then binarize is made to extract the background and the moving objects, i.e., pixel value of the background is set to 0 and pixel value of the moving objects is set to 255.
Then, a copy of this series was changed to a binary picture by a thresholding procedure so that the image of polysterene beads was set to bright and the background was set to dark.
The background was set to the total list of genes expressed in this data set.
The background was set to zero, and the injection start was synchronized (Fig 3b, right panel).
The background was set to the Drosophila_2 array, which corresponds to the Affymetrix GeneChip Drosophila Genome 2.0 array.
The background was set to include only the genes on the qPCR arrays analyzed for each sample group.
Proteins were quantified, using the Volume Rect Tool (Quantity One, Biorad), and the relative volume of the background was set to 1. Lymphoma/leukemia and EOC cell lines were fixed in 4% paraformaldehyde (PFA Thermo Scientificic) for 10 minutes.
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