Sentence examples for background were sequenced using from inspiring English sources
Two progenies (RJW207.1 and RJW207.3) carrying the same secondary metabolite phenotype as the original mutant (e.g. restored NOR production in a ΔlaeAΔstcEveA1 background) were sequenced using Illumina technology.
Plasmids isolated were sequenced using Sanger sequencing.
Libraries were sequenced using Illumina Hi-Seq platforms.
Mutants are crossed into other backgrounds over many generations until a small area is fine mapped and can be sequenced using more traditional means, including Sanger sequencing.
More than 14.4 kbp of the rDNA covering most of the transcribed region for primary rRNA in A5 lung cells of BALB/c mouse background were sequenced (GenBank GU372691) using primers selected from our assembled C57BL6 mouse sequence (see Materials and Methods).
Positive clones having signals greater than 10x background were sequenced, amino acid sequences predicted using BioEdit [84] and unique clones identified using Multalin [85].
All plasmids were sequenced before use.
Recombinants were sequenced before use.
Partial plasmid sequences were obtained by sequencing using MySeq platform.
After staining, the blots with Coomassie Brilliant Blue ELP protein bands, appearing white on a blue background, were used for N-terminal sequencing by Midwest Analytical (St . Louis MO, USA).
Input was sequenced from nontransgenic (wt) and Hairy overexpressing embryos and used as background.
