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For investigating PMM chromosomal location, the PMM gene specific fragments, amplified from CS, the different NT lines of CS, LDN, and the D genome substitution lines in LDN background, were separated using CE and differentiated by fragment analysis.
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For each slide the foreground and background signal intensities were separated using the irregular feature-finding algorithm implemented in the software.
Supernatants were separated using centrifugation.
In addition, the background signals were separated from those of the model compound.
At first, heart images were separated from the background using GrabCut method.
Immunolabeled vessels were separated from the background using the threshold tool, and the average pixel intensity (brightness value) of the immunofluorescent signal was measured.
For determining optical properties in the presence of absorption, the absorption effects are separated out using a background scattering model with a power-law scattering dependence on wavelength [ 9, 11, 13], so that Eq. 1 can be applied.
Muscle artefacts were separated from a contaminated segment of background activity using BSS-CCA [7].
Then, background corner points are separated from foreground corner points using an estimate of the motion vector based on block matching between adjacent frames: points whose motion speed is under a threshold are not considered for further analysis.
In brief, the plant was separated from the imaging background using a nearest-neighbour colour classification.
SHG-B was detected at Ex 800 and 890 nm (Additional file 11: Figure S9B), but not at Ex 735 nm (not shown), and could be separated from the background autofluorescence by using the lambda scan to generate narrow bandwidth images (Additional file 11: Figure S9B, green arrows and SHG-B images).
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