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The minimum frequency to benchmark selection of peaks was 25%, and only peaks with a signal/noise intensity above background were selected by the software.
The pixels showing hypointensity in contrast to the background were selected by thresholding at the lowest 25%% of the signal intensities and used for measurement.
A more stringent analysis was performed where in addition to a cut-off >2-fold and p-value < 0.05, probe sets with expression levels >3-fold higher than background were selected; by this approach, we identified 132 downregulated and 183 upregulated probe sets in CD16+ compared to CD16- Mo (data not shown).
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T1 transgenic plants harboring the construct in Col-0 and irx8-5 homutantte mutant backgrounds were selected by genotyping PCR (Additional file 5) and used for complementation analyses.
Analysis of the co-localization of endogenous GluA1 with TfR was carried out by background subtracting and thresholding image fields so that only puncta that were twofold greater than background were selected.
Drp-1 punctae were selected by using a threshold on background corrected images and were quantified.
Mitochondria were selected by using defined thresholds post background correction and were quantified by using Metamorph (Molecular Devices Corporation).
Key terms were selected by the authors from the literature pertaining this study's theoretical background and prior research.
Joe Hart, John Terry, Mario Balotelli and Carlos Puyol were selected by only one respondent.
The other four were selected by Mr. Giuliani.
If you are adjusting the brightness of an image that hasn't been split into layers, the single layer "Background" will be selected by default.
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CEO of Professional Science Editing for Scientists @ prosciediting.com