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Progeny containing CyO, P{ry[+t7.2] = sevRas85D V12 }FK1 and either heterozygous mutant candidate genes or wild-type (w 1118 ) background were scored for rough eye phenotypes.
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After normalization for the intensity of background staining, at least four areas for each slide were scored for covering of the epidermal layer and hair follicles.
Staining intensities above background levels were scored as positive.
Cells were scored positive for Hoechst, if the integrated pixel intensity was 210-fold above local background and positive for p27 when staining was 30-fold above background (signal obtained with secondary antibody).
Nodules were scored according to degree of FDG uptake (above-background, at-background or below-background).
Only bands between 100 and 1650 bp were scored, as this gel region produced the highest quality profiles, allowing for discrimination of major bands against a low background.
The latter nodule was scored as below-background on OSEM, and background on BPL.
Z-scores for the entire wild type population for each genetic background were normalized for each test using the equation (individual-population mean)/standard deviation.
In contrast to the correlation coefficients, no substantial background was observed for Z-scores and ranking scores (fig. 2 C and D).
Ki67 was visually scored for percentage of tumor cell nuclei with positive immunostaining above the background level by two pathologists.
Ki-67 was visually scored for percentage of tumor cell nuclei with positive immunostaining above the background level.
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