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First, 2-D Fn patterns on glass with protein islands and a cell resistant background were produced by the MAPL technique [39] in order to determine whether the surface area of cell adhesion, which was shown to influence cell contractility [40], also impacts rearrangement of surface adsorbed Fn into fibrils.
Mice, maintained on a 129/SvEvBrd background, were produced by heterozygote × heterozygote crosses and genotyped by Southern blot or polymerase chain reaction analysis of tail biopsy DNA.
WSX-1 transgenic mice in the MRL/ lpr background were produced by crossing WSX-1 transgenic BALB/c mice 24 into the MRL/ lpr background more than six times (continual backcrossing: 98.44% in MRL/ lpr background).
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The background is produced by the AR model with an autoregression factor of 0.2.
A quantitative measure of the difference between distributions associated with each MW versus the background was produced by the Kolmogorov-Smirnov test, yielding a p-value for each MW.
Although some background was produced by the new method it was greatly reduced compared to the unmodified method (Fig. 6, compare blue to red).
Based upon analysis of the sequences of the DNA from the junctions of the vector LTR and cellular chromosomes, these rare integrated NIL vector sequences were not mediated by an integrase-driven mechanism due to reversion of the engineered mutations, but more likely were produced by background recombination events.
Inspection of the original scanned image revealed that these outliers were produced by a faulty background subtraction method that extracted signals from the scanned image.
Transgenic worms were produced by microinjection of the resulting plasmid into the unc-119 ed3 unc-119 ed3 and the unc-119(ed3) and glo-4(ok623) background.
Input models were produced by alternatingly placing cells with a ±20%% perturbation from the background velocity.
All three shows were produced by Paul Henning.
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