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The fluorescence signals of tumors relative to background were measured using Image J software (version 1.47 for Windows) and results were presented as fold changes vs. Background.
The signal intensities of Cy5 and Cy3 dyes in each spot and the local background were measured using the GenePix 4000B microarray scanner (Axon Instruments, USA) to calculate the net intensity of each spot for analysis.
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The number of photo-stimulated luminescence events per square millimeter (PSL/mm2, mean ± SD) corrected for background was measured using vendor-specific software (BAS-Reader) by drawing individual regions of interest (ROIs) for the aortic wall and psoas muscle, facilitated by comparison with block face photos for each section.
The background was measured using the 'corners between spots' protocol of the software and was deducted from the 'volumes'volumes
The intensity of the fluorescent protein signal in the localization region of interest relative to the background was measured using Metamorph software (Molecular Devices).
Fluorescence intensities of the bleached, unbleached, and background areas were measured using Image J 1.46r.
The signal intensities of the spots and background values were measured using array vision 7.0 (array vision, USA).
Average den-sities of the bands above background levels were measured using a BioRad GS-800 Calibrated Densitometer using Quantity One 4.6.4 software.
Average intensities in all ROIs including the background signal were measured using ImageJ 1.44p software (http://imagej.nih.gov/ij), before exporting data into Microsoft Excel 2007 (http://www.microsoft.com).
Slides were scanned on a confocal laser scanner (Arrayexpress; Packard Bioscience, USA) and, for each spot, signal and background intensities were measured using histogram method of Quantarray software (version 3.0, Packard BioScience, BioChip Technologies LLC, USA).
Background radioactivity levels were measured using a handheld intraoperative gamma detector/probe (programmed to read out in counts per second) at the skin surface well away (not less than 20 cm) from the injection site.
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