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Univariate comparisons by rural vs urban background were made using the χ test for categorical variables and unpaired T-test for each selection factor.
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The calibration curve and background corrections were made using the same procedure as those from the NanoSIMS analysis.
Fine adjustments to the layout (e.g. font colours, background colours) were made using Adobe Illustrator.
To obtain a baseline background current, measurements were made using the same recording solution supplemented with 5 mM BaCl2.
It is worth noting, however, that these measurements of background particulate counts were made using diluent in the absence of protein.
Cube, background, and palette chromaticity measures were made using a Spectrascan PR-655 spectroradiometer (Photo Research Inc, Chattsworth, CA) and are reported in Allred and Olkkonen (2013).
Base calls were made using the ABACUS (adaptive background genotype calling scheme) algorithm [31].
The readings were made using a 630 nm (background) and a 570 nm filter.
Fluorescence measurements were made using flow cytometry throughout growth in wild-type, Δ sigB and Δ rsbV backgrounds.
An external check on the background can be made using the ROSAT All-Sky Survey R4-R5 band images.
Background correction was made using a reference blank KBr pellet.
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