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mpk4 [5], mks1 (GT.108403; [8]) and the mpk4/mks1 double mutant, all in Landsberg (Ler) background, were grown in chambers under short-day (8 h light/16 h darkness).
BAS1-GUS fusions in the bas1-2 sob7-1, and phyB-9 background7-1 background were grown in continuous red light for 5 days.
Three independent pools of homozygous yeast deletion mutants (BY4743 background) were grown in YPD media in the presence of BPS at concentrations of 75 and 100 μM.
Wt and ubr2Δ harboring the HSP104-GFP reporter in the SGA background were grown in YPD 2% glucose to mid-exponential phase and treated with 0.6 mM H2O2 and samples were taken over time.
'Dong WT (WT) and the GMS mutant in the 'Dong A' background were grown in an experimental field at the China Agricultural Academy of Science Cotton Research Institute under standard field conditions during the spring and summer of 2010.
The normal maize inbred A69Y wt) and the endosperm mutant genotypes o2, o7, and o2o7, in a near-isogenic A69Y background were grown in adjacent plots in the genetic nursery of the Maize Research Unit in Bergamo (Italy), during summer 2006.
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The mating tester BE287α (ura4 car2 gal2), the wild type reference strain (BY4742) and the deletion strains (BY4742 background) were grown overnight in YPD medium at 30°C.
Plants of Columbia background were grown at 23°C in long days.
Growth of S. aureus RN4220 (a gift from Prof. Simon Foster, University of Sheffield), and gene disrupted strains made in this background, were grown to mid-exponential phase in 10 ml TSB and diluted to a starting OD600 of 0.001 in 100 μl TSB in a 96-well plate (Greiner).
The two phylogeographically distinct P. australis clones (DK clone, European genetic background; ALG clone, Mediterranean genetic background) were grown for 151 days in phytotrons at 19/12 °C (day/night temperature) and 390 ppm CO2, and at elevated temperature (+5 °C) and CO2 (700 ppm) with treatment factors alone or in combination.
Yeast cells (BY4742 strain background) were grown to exponential phase (A600 ∼0.6) in YPD medium without or with arsenite (1.5 mM sodium arsenite, 1 hour) and equivalent cell numbers (10 A600 units) were used to isolate aggregated proteins as described previously (Jacobson et al., 2012; Rand and Grant, 2006).
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