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VEGF-B deficient rats of Sprague-Dawley background were generated with a zinc-finger nuclease based technique by Sigma Advanced Genetic Engineering Labs, Sigma-Aldrich Biotechnology (St .Louis, Missouri, USA).
To explore the mechanisms and physiological significance of KIF17, transgenic mice with different genetic background were generated, including wild-type KIF17 (TgS), KIF17 with S1029A (TgA), or KIF17 with S1029D (TgD) phosphomimic mutations [ 80].
Other double mutants, including ste12ssn8, ste20ssn8, cpk1ssn8, and SSN8 overexpression in the cwc1 mutant background, were generated by crossing the strains with appropriate genotypes.
Mice with floxed alleles for Rps6kb1 on a C57Bl/6 background were generated by Taconic Biosciences and crossed with the Cre-expressing transgenic mice to generate compound heterozygotes.
The tku70Δ and tmus53Δ mutants in the CBS999.97 background were generated by crossing each QM6a mutant with the wild isolate CBS999.97 1-1, re), respectively.
Heterozygous PS1xAPP double transgenic mice (C57BL 6 background) were generated by crossing homozygous PS1 transgenic mice with heterozygous Thy1-APP751SL mice.
Heterozygous PS1xAPP double transgenic mice (C57BL/6 background) were generated by crossing homozygous PS1 transgenic mice with heterozygous Thy1-APP751sl ones.
The data for the M6 mouse breast cancer cell line, other C3(1)/Tag mouse mammary tumors and normal mammary tissue samples (FVB background) were generated using Affymetrix MOE430A arrays and processed with RMA and quantile normalization [ 15].
Dgat1−/− mice (C57BL/6J background) were generated as described [ 8].
E1-DN mice in FVB background were generated previously [ 31].
HBx transgenic mice (C57B/6 background) were generated as described.
