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RARα1-/ mice (129/Bl-6 129/Bl-6nd) were generated in Pierre Chambackgroundratory (IGBMC, Strasbourg, France), and MMTV- were mice (FVB, SJL, and Bl/6 mixed backgeneratedinh FVB Pierre Chambonre generously provided by Dr. Yi Li (Baylor College of Medicine, Houston, TX, USA).
SOD1G93A mice on a B6 background were generated by crossing B6SJL-TgN SOD1-G93A 1Gur males (Jackson Laboratory) to B6 females for 4 7 generations (N4 N7), and then SOD1G93A mice on an Als2-null background were generated in a similar manner.
Briefly, transgenic mice in the C57BL6 strain background were generated in which the gene construct harbours the human CBS with the mis-sense mutation 833 T>C.
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Transient focussed wave groups, and embedded wave groups in a smaller regular wave background are generated in a towing tank, and the jacket is towed under different speeds opposite to the wave direction to simulate wave loading with different in-line uniform currents.
The 21D-GAL4 strains with null mutation of gene period (per 01 21D/TM2) or altered cryptochrome (cryb 21D/TM6B) backgrounds were generated in Dr. Michael Rosbash' laboratory (HHMI, Brandeis Univ., USA) (Fig. 2).
Floxed GluR2 mice in a 129S6 background, were generated as described in Text S1.
The monogenic lines of QTL1 and QTL11 of JHN in the CO39 genomic background were generated as illustrated in Additional file 2: Figure S1.
Point mutations of IME2 in the SK1 background were generated by 2-step loop-in, loop-out gene replacement technique using selection and counter-selection of the URA3 marker at the IME2 genomic locus.
RAGE-deficient (rage −/− ) [ 11] and tlr4 −/− mice [ 12], backcrossed >10 times to a C57BL/6 background were generated as described and bred in the animal facility of the Academic Medical Center (Amsterdam, the Netherlands).
E1-DN mice in FVB background were generated previously [ 31].
APChigh-mice on a C57BL/6 genetic background were generated as described [ 21] and bred in the animal facility of the Academic Medical Center in Amsterdam.
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